Elucidando a expressão gênica de enzimas em fungos filamentosos para produção eficiente de energia de biomassa
Os fungos filamentosos há muito são bons amigos dos fabricantes de saquê, mas em breve também poderão ser um ajudante dos ambientalistas. Pesquisadores da Universidade Metropolitana de Osaka revelaram os mecanismos reguladores...
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)
Uma imagem de microscopia eletrônica de varredura do fungo filamentoso A. aculeatus e um modelo de regulação gênica em A. aculeatus. Crédito: Shuji Tani, Universidade Metropolitana de Osaka
Os fungos filamentosos há muito são bons amigos dos fabricantes de saquê, mas em breve também poderão ser um ajudante dos ambientalistas. Pesquisadores da Universidade Metropolitana de Osaka revelaram os mecanismos reguladores da produção de enzimas em um fungo filamentoso que permite a degradação eficiente da biomassa vegetal, um recurso energético alternativo ao petróleo.
Os resultados da pesquisa foram publicados na Applied Microbiology and Biotechnology em 10 de janeiro de 2023.
Fungos filamentosos (bolores) são microorganismos com uma longa história de uso na fermentação de saquê, molho de soja, queijo e muitos outros produtos. Essa fermentação é um bom exemplo do uso industrial da capacidade dos fungos filamentosos de secretar várias enzimas em grandes quantidades.
Atualmente, a biomassa vegetal está chamando a atenção como uma alternativa ao petróleo, que eventualmente se esgotará. Como as paredes celulares duras das plantas são compostas por vários aromáticos e polissacarídeos, sua degradação requer um grande número de enzimas com características diversas. Consequentemente, estudos têm sido conduzidos para utilizar fungos filamentosos como uma importante fonte de enzimas para a degradação da biomassa vegetal.
Aprofundando-se nesse campo, uma equipe de pesquisa liderada pelo professor associado Shuji Tani, da Graduate School of Agriculture da Osaka Metropolitan University, analisou os mecanismos reguladores da produção de enzimas hidrolisadoras de carboidratos no fungo filamentoso Aspergillus aculeatus, que produz enzimas com excelente capacidade de degradar a biomassa vegetal.
A uridina difosfato (UDP)-glicose 4-epimerase (Uge5) é bem conhecida como uma enzima envolvida no metabolismo da galactose. No entanto, a equipe descobriu que Uge5 também regula a expressão de genes de enzimas degradantes em A. aculeatus. Este é o primeiro relato dos papéis de Uge5 na expressão seletiva de genes em resposta a diferentes tipos de açúcares indutores em fungos filamentosos.
Essas descobertas abordam o atual desafio tecnológico em estabelecer um método de alta produção abrangente e muito desejado para várias enzimas em fungos filamentosos .
O professor Tani explicou: "Construímos e analisamos uma biblioteca contendo aproximadamente 9.000 cepas de Aspergillus aculeatus com alteração genética e identificamos Uge5 como um novo fator regulador que regula a produção de enzimas de hidrólise de carboidratos. A descoberta dessa nova função nos pegou de surpresa . Planejamos continuar nossa pesquisa para elucidar fenômenos que o conhecimento existente não pode explicar."
Mais informações: Uma nova função de uma UDP-glicose 4-epimerase putativa na expressão de genes de glicosídeo hidrolase em Aspergillus aculeatus, Applied Microbiology and Biotechnology (2023). DOI: 10.1007/s00253-022-12337-8
Informações da revista: Microbiologia Aplicada e Biotecnologia