As modulaa§aµes na estrutura do biofilme como resultado demudanças de temperatura são reguladas pela produção de uma nova proteana extracelular chamada BsaA na bactanãria C. perfringens.
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)
As bactanãrias tem a capacidade de se adaptar ao seu ambiente para sobreviver a defesa imunola³gica do hospedeiro. Uma dessas estratanãgias de sobrevivaªncia inclui a formação de um biofilme que impede que o sistema imunológico ou antibia³ticos cheguem a s bactanãrias. Em um novo estudo, pesquisadores da Universidade de Tsukuba revelaram que as modulações na estrutura do biofilme como resultado demudanças de temperatura são reguladas pela produção de uma nova proteana extracelular chamada BsaA na bactanãria C. perfringens.
C. perfringens vive em vários ambientes, no solo e no intestino dos animais, e pode causar intoxicação alimentar, gangrena gasosa e diarranãia associada a antibia³ticos. a‰ uma bactanãria anaera³bica que não écapaz de crescer fora de um hospedeiro devido a presença de oxigaªnio. Embora seja de conhecimento geral que ele pode se transformar em esporos para evitar ataques ambientais, não foi atérecentemente que C. perfringens também demonstrou ter a capacidade de formar biofilmes. Nesses biofilmes, uma comunidade de bactanãrias C. perfringens se cobre em uma matriz densa das chamadas substâncias polimanãricas extracelulares (EPS) - que contem proteanas, a¡cidos nuclanãicos e moléculas de açúcar -, protegendo-se de riscos externos. Atéo momento, ainda não estãoclaro como o C. perfringens estãousando biofilmes para sobreviver em ambientes ricos em oxigaªnio.
"Já mostramos que a temperatura éuma sugestãoambiental que influencia a morfologia do biofilme de C. perfringens ", diz o autor do estudo, Professor Nobuhiko Nomura. "Embora em temperaturas mais altas, como 37 ° C, as bactanãrias se prendam a ssuperfÍcies e se acumulem densamente em um biofilme aderente, a temperaturas mais baixas elas constroem um biofilme mais espesso e semelhante a uma pelacula. Queraamos saber como eles são capazes de modular a estrutura. do biofilme em resposta amudanças de temperatura ".
Para atingir seu objetivo, os pesquisadores construaram uma biblioteca de 1.360 células mutantes (nocaute genanãtico) em C. perfringens para ver quais proteanas são necessa¡rias para formar um biofilme semelhante a uma pelacula a 25 ° C. Durante a triagem, eles notaram a presença de uma nova proteana chamada BsaA, produzida dentro da bactanãria e transportada para o exterior. Sem BsaA, as bactanãrias formavam um biofilme de pelacula fra¡gil ou apenas um biofilme aderente. Os pesquisadores mostraram que várias proteanas BsaA se reaºnem em um polamero fora das células para permitir a formação de um biofilme esta¡vel. Quando expostas ao antibia³tico penicilina G ou oxigaªnio, C. perfringens sem BsaA teve uma taxa de sobrevivaªncia significativamente reduzida em comparação com C. perfringens normal.
"Nossos resultados mostram que o BsaA énecessa¡rio para a formação de biofilme semelhante a uma pelacula a 25 ° C e confere tolera¢ncia a antibia³ticos", diz o principal autor do estudo, professor Nozomu Obana. "Sabemos que os biofilmes contem populações celulares heterogaªneas, o que leva a comportamentos multicelulares. Portanto, queraamos saber se a heterogeneidade celular afeta a produção de BsaA e, portanto, a formação de um biofilme semelhante a uma pelacula".
Os pesquisadores descobriram que a proteana SipW controla a polimerização de BsaA em um biofilme, e usou isso para estudar a formação de biofilme. Ao construir C. perfringens que produzia uma proteana fluorescente quando o SipW éproduzido, permitindo que essas células fossem rastreadas por microscopia fluorescente, os pesquisadores conseguiram mostrar que nem todas as bactanãrias produziam SipW. Além disso, eles descobriram que a população de bactanãrias produtoras de SipW começou a cair significativamente quando a temperatura aumentou de 25 ° C para 37 ° C. Curiosamente, a 25 ° C, as células que não produzem SipW estavam localizadas próximas a superfÍcie da bactanãriaestavam sentados e cobertos por células produtoras de SipW. A produção heterogaªnea de SipW e, portanto, BsaA, pode, portanto, garantir que as células com maior tolera¢ncia a riscos externos protejam a subpopulação bacteriana em risco.
"A 25 ° C, émais prova¡vel que C. perfringens seja exposto a tensaµes externas. Nossos resultados fornecem uma explicação de como uma comunidade de C. perfringens garante que ela permanea§a protegida quando a temperatura muda. Nosso estudo ajuda a entender as propriedades do biofilme e fornece informações sobre o desenvolvimento de novas estratanãgias antibacterianas ", diz o professor Nomura.
O artigo "Expressão gaªnica da matriz extracelular heterogaªnea regulada por temperatura define a morfologia do biofilme em Clostridium perfringens" foi publicado na npj Biofilms and Microbes .