Proteanas coreografam a dança infinitesimal de células vivas e biomateriais funcionais
De acordo com um artigo publicado hoje na Scientific Reports , as caracteristicas e propriedades em nanoescala de um substrato subjacente não afetam a resposta biológica das células diretamente.
![](data:image/jpeg;base64,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)
Microestruturas "semelhantes a flores" formando-se sobre um mona³lito de vidro nanoporoso com poros de 31 nm de dia¢metro. Crédito: Lehigh University, Thamma, Kowal, Falk, Jain
Uma equipe de pesquisa interdisciplinar da Lehigh University desvendou como os biomateriais funcionais dependem de uma camada de proteana interfacial para transmitir sinais a s células vivas sobre sua adesão, proliferação e desenvolvimento geral.
De acordo com um artigo publicado hoje na Scientific Reports , as caracteristicas e propriedades em nanoescala de um substrato subjacente não afetam a resposta biológica das células diretamente. No entanto, essas propriedades influenciam indiretamente o comportamento celular por meio de seu controle sobre as proteanas adsorvidas.
No artigo, "A nanoestrutura do vidro bioativo afeta a fixação das células ósseas por meio da reestruturação da proteana após a adsorção", a equipe de Lehigh demonstra que as células vivas respondem a s caracteristicas da camada interfacial que surgem como consequaªncia de estruturas em escala micro e nano projetadas em um material de substrato . Essas estruturas infinitesimalmente minaºsculas tem um enorme impacto sobre a natureza das proteanas e como elas se reestruturam e interagem eletrostaticamente com o material, o que por sua vez influencia a maneira como as células se fixam ao substrato e se desenvolvem ao longo do tempo.
"Ha¡ outros que estudaram a camada de proteana interfacial ", diz Himanshu Jain, o TL Diamond Distinguished Chair em Engenharia e Ciências Aplicadas e Professor de Ciência de Materiais e Engenharia em Lehigh, que também atua como diretor do Instituto de Materiais e Dispositivos Funcionais de Lehigh (I-FMD). "Mas este trabalho mostrou direta e inequivocamente pela primeira vez como algumas caracteristicas especaficas em nanoescala do substrato podem impactar a estrutura molecular secunda¡ria da interface proteinada que por sua vez afeta a resposta das células que são milhares de vezes maiores."
Matthias Falk, professor de Biologia Celular da Lehigh's College of Arts and Sciences, se juntou ao professor Jain na orientação desta pesquisa. A equipe écompletada por dois alunos de doutorado supervisionados conjuntamente por Falk e Jain - Dr. Tia Kowal, que recebeu Ph.D. em Ciências Biola³gicas e agora éum pesquisador de pa³s-doutorado na Medicina de Stanford, e o autor principal Dr. Ukrit Thamma, que concluiu seu doutorado em Ciência e Engenharia de Materiais e agora éprofessor da Universidade de Tecnologia King Mongkut em Bangkok, Taila¢ndia.
"Lehigh écada vez mais reconhecido como um lugar onde a ciência da equipe interdisciplinar estãose enraizando e florescendo", diz Jain. "A criação e a missão dos Institutos de Pesquisa Interdisciplinar de Lehigh éuma expressão estratanãgica dessa noção - e este projeto éuma expressão dessa noção em ação. E o papel crucial que nossos alunos desempenham, com o apoio de uma ampla equipe de professores, fala por si são . "