A nitrogenase dependente de vana¡dio pode ligar duas moléculas de CO simultaneamente
Os pesquisadores recentemente apresentaram seus resultados na revista Science Advances .
![](data:image/jpeg;base64,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)
O cofator ferro-vana¡dio (FeV) na nitrogenase dependente de vana¡dio foi feito para reagir com o mona³xido de carbono (CO) e então gaseificado sob pressão, permitindo que duas moléculas do substrato fossem visualizadas na forma ligada. O cofator FeV éum dos maiores e mais complexos centros meta¡licos em proteanas atualmente conhecidos. Ele consiste em sete aons de ferro (cinza), 9 aons de enxofre (amarelo), um carbono central (preto) e um aon vana¡dio (verde), e também carrega um aon carbonato e uma molanãcula de homocitrato como ligantes orga¢nicos. Crédito: Oliver Einsle
Por meio da fixação biológica do elemento nitrogaªnio pela enzima nitrogenase, os organismos obtem acesso ao nitrogaªnio molecular (N 2 ) da atmosfera terrestre, essencial para a construção de estruturas celulares. Além disso, uma variante da nitrogenase dependente do vana¡dio pode reduzir o gás ta³xico mona³xido de carbono (CO) a hidrocarbonetos. Essas reduções de N 2 e CO estãoentre os processos mais importantes da química industrial, pois são usados ​​para produzir fertilizantes e combustaveis sintanãticos. No entanto, os pesquisadores ainda não foram capazes de decifrar as diferentes vias das duas reações.
O Dr. Michael Rohde da equipe do Prof. Dr. Oliver Einsle no Instituto de Bioquímica da Universidade de Freiburg, em colaboração com dois grupos de pesquisa na Freie Universita¤t Berlin, conseguiu mostrar como o satio ativo da nitrogenase dependente de vana¡dio écapaz de ligar duas moléculas de CO simultaneamente, criando assim a base para combinar os a¡tomos de carbono espacialmente adjacentes de ambas as moléculas em um processo redutivo. Os pesquisadores recentemente apresentaram seus resultados na revista Science Advances .
As reduções industriais de N 2 e CO - conhecidas como processos Haber-Bosch e Fischer-Tropsch, respectivamente - exigem altas temperaturas e pressão. Enquanto a redução do N 2 leva ao produto biodisponavel de ama´nio (NH 4 + ), pelo menos dois a¡tomos de carbono se combinam durante a conversão de CO. O produto de reação predominante éo etileno (eteno, C 2 H 4), um gás incolor que desempenha um papel importante não são nos combustaveis, mas também na produção de pla¡sticos. Embora a clivagem de uma ligação NN na fixação de nitrogaªnio seja quimicamente fundamentalmente diferente da formação de uma ligação CC na redução de CO, os cientistas suspeitaram anteriormente que a nitrogenase usa os mesmos princapios mecanasticos ba¡sicos para ambas as reações.
Em um trabalho anterior, a equipe liderada por Rohde e Einsle usou nitrogenase para reagir com o gás CO, resultando na ligação especafica de uma única molanãcula. Em seu estudo atual, que se baseia neste trabalho, os pesquisadores mostram que gaseificaram cristais desse primeiro estado com CO sob pressão e, em seguida, os submeteram a análise cristalogra¡fica de raios-X. Isso permitiu que observassem diretamente como uma segunda molanãcula de CO se liga. “A forma de nitrogenase obtida desta forma, com duas moléculas de CO no satio ativo, provavelmente representa um estado bloqueado ", explica Rohde," mas fornece pistas diretas para o mecanismo da enzima. "Como resultado, a equipe de Einsle pode agora delinear um mecanismo detalhado de redução do CO por meio da nitrogenase.